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Literature summary for 1.13.12.19 extracted from

  • Nagahama, K.; Yoshino, K.; Matsuoka, M.; Tanase, S.; Ogawa, T.; Fukuda, H.
    Site-directed mutagenesis of histidine residues in the ethylene-forming enzyme from Pseudomonas syringae (1998), J. Ferment. Bioeng., 85, 255-258.
No PubMed abstract available
Search for more literature for 1.13.12.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas syringae

Protein Variants

Protein Variants Comment Organism
H116Q kcat value decreases to 2.4% of wild-type. Mutant is more thermolabile than wild-type Pseudomonas syringae
H168Q kcat value decreases to 3% of wild-type. Mutant is more thermolabile than wild-type Pseudomonas syringae
H169Q kcat value decreases to 9.3% of wild-type. Mutant is more thermolabile than wild-type Pseudomonas syringae
H189Q complete loss of activity Pseudomonas syringae
H233Q complete loss of activity Pseudomonas syringae
H268Q kcat value decreases to 1.8% of wild-type Pseudomonas syringae
H284Q kcat value decreases to 2% of wild-type. Mutant is more thermolabile than wild-type Pseudomonas syringae
H305Q kcat value decreases to 40% of wild-type Pseudomonas syringae
H309Q kcat value decreases to 3.3% of wild-type. Mutant is more thermolabile than wild-type Pseudomonas syringae
H335Q kcat value decreases to 60% of wild-type Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 2-oxoglutarate mutant H305Q, pH 8.0, 25°C Pseudomonas syringae
0.007
-
 2-oxoglutarate mutant H168Q, pH 8.0, 25°C Pseudomonas syringae
0.007
-
 2-oxoglutarate mutant H284Q, pH 8.0, 25°C Pseudomonas syringae
0.008
-
 2-oxoglutarate mutant H335Q, pH 8.0, 25°C Pseudomonas syringae
0.01
-
 2-oxoglutarate mutant H309Q, pH 8.0, 25°C Pseudomonas syringae
0.011
-
 2-oxoglutarate mutant H169Q, pH 8.0, 25°C Pseudomonas syringae
0.013
-
 2-oxoglutarate wild-type, pH 8.0, 25°C Pseudomonas syringae
0.015
-
 2-oxoglutarate mutant H116Q, pH 8.0, 25°C Pseudomonas syringae
0.033
-
 2-oxoglutarate mutant H268Q, pH 8.0, 25°C Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae P32021 pv. phaseolicola PK2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + O2
-
 Pseudomonas syringae ethylene + 3 CO2 + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 2-oxoglutarate mutant H268Q, pH 8.0, 25°C Pseudomonas syringae
0.01
-
 2-oxoglutarate mutant H284Q, pH 8.0, 25°C Pseudomonas syringae
0.012
-
 2-oxoglutarate mutant H116Q, pH 8.0, 25°C Pseudomonas syringae
0.015
-
 2-oxoglutarate mutant H168Q, pH 8.0, 25°C Pseudomonas syringae
0.017
-
 2-oxoglutarate mutant H309Q, pH 8.0, 25°C Pseudomonas syringae
0.047
-
 2-oxoglutarate mutant H169Q, pH 8.0, 25°C Pseudomonas syringae
0.2
-
 2-oxoglutarate mutant H305Q, pH 8.0, 25°C Pseudomonas syringae
0.3
-
 2-oxoglutarate mutant H335Q, pH 8.0, 25°C Pseudomonas syringae
0.5
-
 2-oxoglutarate wild-type, pH 8.0, 25°C Pseudomonas syringae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.27
-
 2-oxoglutarate mutant H268Q, pH 8.0, 25°C Pseudomonas syringae
0.8
-
 2-oxoglutarate mutant H116Q, pH 8.0, 25°C Pseudomonas syringae
1.43
-
 2-oxoglutarate mutant H284Q, pH 8.0, 25°C Pseudomonas syringae
1.67
-
 2-oxoglutarate mutant H309Q, pH 8.0, 25°C Pseudomonas syringae
2.14
-
 2-oxoglutarate mutant H168Q, pH 8.0, 25°C Pseudomonas syringae
4.24
-
 2-oxoglutarate mutant H169Q, pH 8.0, 25°C Pseudomonas syringae
33.3
-
 2-oxoglutarate mutant H305Q, pH 8.0, 25°C Pseudomonas syringae
37.5
-
 2-oxoglutarate mutant H335Q, pH 8.0, 25°C Pseudomonas syringae
38.5
-
 2-oxoglutarate wild-type, pH 8.0, 25°C Pseudomonas syringae