Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas savastanoi |
Crystallization (Comment) | Organism |
---|---|
determined by X-ray diffraction methods to a resolution of 1.95 A. The overall structure of the protein shows that it has the same fold as members of the monoamine oxidase family of flavoproteins, with the greatest similarities to the L-amino acid oxidases | Pseudomonas savastanoi |
Protein Variants | Comment | Organism |
---|---|---|
K365M | mutation decreases the kcat and kcat/Km (L-tryptophan) values by 60000- and 2 millionfold compared to wild-type, respectively | Pseudomonas savastanoi |
W466F | mutation decreases the kcat value less than 2fold and the kcat/Km (L-tryptophan) value only 5fold compared to wild-type | Pseudomonas savastanoi |
W466M | mutation results in an enzyme lacking flavin and detectable activity | Pseudomonas savastanoi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-tryptophan | pH 8.3, 25°C, wild-type | Pseudomonas savastanoi | |
0.13 | - |
L-tryptophan | pH 8.3, 25°C, mutant W466F | Pseudomonas savastanoi | |
1.2 | - |
L-tryptophan | pH 8.3, 25°C, mutant K365M | Pseudomonas savastanoi | |
90 | - |
O2 | pH 8.3, 25°C, wild-type | Pseudomonas savastanoi | |
140 | - |
O2 | pH 8.3, 25°C, mutant W466F | Pseudomonas savastanoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas savastanoi | P06617 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | - |
Pseudomonas savastanoi | indole-3-acetamide + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tryptophan 2-monooxygenase | - |
Pseudomonas savastanoi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas savastanoi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00022 | - |
L-tryptophan | pH 8.3, 25°C, cosubstrate: O2, mutant K365M | Pseudomonas savastanoi | |
9.2 | - |
L-tryptophan | pH 8.3, 25°C, cosubstrate: O2, mutant W466F | Pseudomonas savastanoi | |
13.2 | - |
L-tryptophan | pH 8.3, 25°C, cosubstrate: O2, wild-type | Pseudomonas savastanoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Pseudomonas savastanoi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Pseudomonas savastanoi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
L-tryptophan | pH 8.3, 25°C, mutant K365M | Pseudomonas savastanoi | |
70 | - |
O2 | pH 8.3, 25°C, mutant W466F | Pseudomonas savastanoi | |
72 | - |
L-tryptophan | pH 8.3, 25°C, mutant W466F | Pseudomonas savastanoi | |
140 | - |
O2 | pH 8.3, 25°C, wild-type | Pseudomonas savastanoi | |
360 | - |
L-tryptophan | pH 8.3, 25°C, wild-type | Pseudomonas savastanoi |