Literature summary for 1.13.12.5 extracted from

Cheung, C.Y.; Webb, S.E.; Meng, A.; Miller, A.L.
Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development (2006), Int. J. Dev. Biol., 50, 561-569.

Search for more literature for 1.13.12.5

Application

Application	Comment	Organism
molecular biology	when aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by about 24 hours. Thus, it is not possible to image Ca2+ signals from later stages of zebrafish development using this approach. Transient expression of apoaequorin (i.e., the protein component of aequorin) using aeq-mRNA in zebrafish embryos and then reconstitution of intact aequorin in vivo by loading the coelenterazine cofactor into the embryos separately provides a valuable tool for monitoring Ca2+ signaling during the 2448 h post fertilization period of zebrafish development. Thus, it effectively extends the aequorin-based Ca2+-imaging window by an additional 24 hours	Aequorea forskalea

Organism

Organism	UniProt	Comment	Textmining
Aequorea forskalea	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coelenterazine + O2	-	Aequorea forskalea	?	-	?

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Release 2023.1 (January 2023)