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Literature summary for 1.13.12.5 extracted from

  • Maguire, C.; Deliolanis, N.; Pike, L.; Niers, J.; Tjon-Kon-Fat, L.; Sena-Esteves, M.; Tannous, B.
    Gaussia luciferase variant for high-throughput functional screening applications (2009), Anal. Chem., 81, 7102-7106.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.12.5

Activating Compound

Activating Compound Comment Organism Structure
coelenterazine increase of wild-type Gluc activity at higher substrate concentrations Gaussia princeps
Triton X-100 0.1%, 3.7fold increase of specific activity of wild-tpye Gluc Gaussia princeps

Application

Application Comment Organism
molecular biology sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy Gaussia princeps

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH10B cells and 293T human kidney fibroblasts Gaussia princeps

Protein Variants

Protein Variants Comment Organism
M43I after 120 s of incubation time still 87% residual light emission compared to only 30% of wild-type Gluc, stabilized light emission with detergent Triton X-100, more stable light emission compared to wild-type Gluc when expression in mammalian cells Gaussia princeps
additional information without the signal sequence higher enzyme yield and activity Gaussia princeps

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information secreted Gaussia princeps
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 SDS-PAGE Gaussia princeps

Organism

Organism UniProt Comment Textmining
Gaussia princeps
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gaussia princeps

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 value about 100000000000 relative light units/mg protein, wild-type Gluc, in presence of Triton X-100 Gaussia princeps
additional information
-
 value about 20000000000 relative light units/mg protein, mutant M43I Gaussia princeps
additional information
-
 value about 20000000000 relative light units/mg protein, wild-type Gluc Gaussia princeps
additional information
-
 value about 30000000000 relative light units/mg protein, mutant M43I, in presence of Triton X-100 Gaussia princeps

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2 20 microM, assay at pH 8.0 Gaussia princeps ?
-
 ?

Synonyms

Synonyms Comment Organism
Gaussia luciferase
-
 Gaussia princeps

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Gaussia princeps