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Literature summary for 1.13.12.7 extracted from
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis (2002), Biochim. Biophys. Acta, 1597, 271-279.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases expressed in Escherichia coli | Luciola cruciata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Luciola cruciata | - |
- |
- |
| Photinus pyralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases by ammonium sulfate precipitation, gel filtration and hydroxyapatite column chromatography | Photinus pyralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases is more stable than the wild type enzyme, retaining 75% of the activity after 10 min at 50°C | Luciola cruciata |
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