Application | Comment | Organism |
---|---|---|
medicine | luciferase gene is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. The use of thermostabilized luciferases may allow monitoring of micro-metastses and the early stages of tumor growth | Photinus pyralis |
Cloned (Comment) | Organism |
---|---|
- |
Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
E345K/A215L | half-life at 37°C is 7.36 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
E345K/A215L/I232A/T214A | half-life at 37°C is 75.1 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
E345K/A215L/I232A/T214A/F295L | half-life at 37°C is 82.1 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
E345K/I232A/T214A | half-life at 37°C is 15.5 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
E345K/I232A/T214A/F295L/S420T | half-life at 37°C is 72.4 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
E345K/T214A | half-life at 37°C is 8.5 min, compared to 3.06 min for the wild-type enzyme | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 3.06 min for wild-type enzyme,8.5 min for mutant enzyme E345K/T214A, 15.5 min for E345K/I232A/T214A, 7.36 min for E345K/A215L, 72.4 min for E345K/I232A/T214A/F295L/S420T, 82.1 min for E345K/A215L/I232A/T214A/F295L, 75.1 min for E345K/A215L/I232A/T214A | Photinus pyralis |