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Literature summary for 1.13.12.7 extracted from
- A quantitative and highly sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis (2005), J. Med. Microbiol., 54, 1023-1030.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a quantitative and highly sensitive luciferase-based assay for bacterial toxins, overview | Photinus pyralis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of the cDNA encoding the destabilized enzyme into an adenoviral expression plasmid and transfection of Vero, Hep-2, Chang, A-549, COS-1, and HeLa cells, luciferase expression is linear with respect to viral multiplicity of infection, protein synthesis inhibiting drugs, e.g. shiga toxins of Escherichia coli, diphtheria toxin, Pseudomonas exotoxin A and the plant toxin ricin A, and cycloheximide, reduce bioluminescence respresenting the antiviral activity | Photinus pyralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a quantitative and highly sensitive luciferase-based assay for antiviral toxins, overview | Photinus pyralis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| firefly luciferase | - |
Photinus pyralis |
| luciferase | - |
Photinus pyralis |
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