Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain HMS174(DE3) | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
D436A | 3.4fold increased activity compared to the wild type enzyme | Photinus pyralis |
D436E | 50% activity compared to the wild type enzyme | Photinus pyralis |
D436G | 8.5fold increased activity compared to the wild type enzyme | Photinus pyralis |
D436N | 50% activity compared to the wild type enzyme | Photinus pyralis |
D436S | 2.2fold increased activity compared to the wild type enzyme | Photinus pyralis |
D436V | 2% activity compared to the wild type enzyme | Photinus pyralis |
I147A | 30% activity compared to the wild type enzyme | Photinus pyralis |
I147E | 4% activity compared to the wild type enzyme | Photinus pyralis |
I147F | 1.2fold increased activity compared to the wild type enzyme | Photinus pyralis |
I147G | 3% activity compared to the wild type enzyme | Photinus pyralis |
I147K | 4% activity compared to the wild type enzyme | Photinus pyralis |
I147L | 4.7fold increased activity compared to the wild type enzyme | Photinus pyralis |
I147M | 3.4fold increased activity compared to the wild type enzyme | Photinus pyralis |
I147Q | 70% activity compared to the wild type enzyme | Photinus pyralis |
I147R | 1% activity compared to the wild type enzyme | Photinus pyralis |
I147S | 7% activity compared to the wild type enzyme | Photinus pyralis |
I147V | 1.3fold increased activity compared to the wild type enzyme | Photinus pyralis |
L530A | 20% activity compared to the wild type enzyme | Photinus pyralis |
L530D | 1% activity compared to the wild type enzyme | Photinus pyralis |
L530F | 10% activity compared to the wild type enzyme | Photinus pyralis |
L530H | 1.4fold increased activity compared to the wild type enzyme | Photinus pyralis |
L530I | shows wild type enzyme activity | Photinus pyralis |
L530K | 3.2fold increased activity compared to the wild type enzyme | Photinus pyralis |
L530R | 5.1fold increased activity compared to the wild type enzyme | Photinus pyralis |
L530S | 1% activity compared to the wild type enzyme | Photinus pyralis |
L530V | 50% activity compared to the wild type enzyme | Photinus pyralis |
L530Y | 2% activity compared to the wild type enzyme | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
D-luciferin | mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.0037 | - |
D-luciferin | mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.0043 | - |
D-luciferin | mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.009 | - |
ATP | mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.0211 | - |
D-luciferin | wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.0456 | - |
ATP | mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.0538 | - |
ATP | mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis | |
0.1602 | - |
ATP | wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | Q27758 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose 6 Fast Flow column chromatography | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Photinus pyralis | isoelectric focusing | - |
6 |