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Literature summary for 1.13.12.7 extracted from

  • Mehrabi, M.; Hosseinkhani, S.; Ghobadi, S.
    Stabilization of firefly luciferase against thermal stress by osmolytes (2008), Int. J. Biol. Macromol., 43, 187-191.
    View publication on PubMed
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Photinus pyralis

General Stability

General Stability Organism
sorbitol, proline and sucrose with strong stabilizing effect on firefly luciferase activity at 35°C Photinus pyralis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 SDS-PAGE Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose, yield of 95% Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
luciferin + O2 + ATP assay at pH 7.8 Photinus pyralis ?
-
 ?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
 Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Photinus pyralis
30
-
 in presence of sucrose, proline or sorbitol Photinus pyralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 no activity at 40°C Photinus pyralis
35
-
 about 70% loss of activity within 10 min, no residual activity after 60 min, in presence of osmolytes higher residual activity after 60 min: 90% with 1.5 M sucrose, 50% with 1.5 M sorbitol, 35% with 1.5 M proline Photinus pyralis