Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL-21 | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
R213M | Cm (M): 1.8 (wild-type: 2.4), T1/2 (min): 19.5 (wild-type: 19.5), Tm (°C): 46.5 (wild-type: 46.5), activation energy (kcal/mol): 2.59 (wild-type: 6.51), [urea]50% (M): 2.86 (wild-type: 1.35) | Photinus pyralis |
R337Q | Cm (M): 1.7 (wild-type: 2.4), T1/2 (min): 22.1 (wild-type: 19.5), Tm (°C): 50.7 (wild-type: 46.5), activation energy (kcal/mol): 3.39 (wild-type: 6.51), [urea]50% (M): 2.18 (wild-type: 1.35) | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
luciferin | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
stability remains constant upon 120 min | Photinus pyralis |
30 | - |
T1/2 (min): 16.2 (wild-type), 19.5 (mutant R213M), 22.1 (mutant R337Q) | Photinus pyralis |
37 | - |
high loss of activity in less than 10 min | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Photinus pyralis |