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Literature summary for 1.13.12.7 extracted from

  • Priyanka, B.; Rastogi, N.; Raghavarao, K.; Thakur, M.
    Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction (2012), Process Biochem., 47, 1358-1363.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Photinus pyralis
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Purification (Commentary)

Purification (Comment) Organism
a more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE) is designed. Downstream processing of luciferase from Photinus pyralis is carried out using polymer/salt aqueous two phase system (ATPS) at 4°C. The enzyme is observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) are observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21 Photinus pyralis

Synonyms

Synonyms Comment Organism
luciferase
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Photinus pyralis