Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE) is designed. Downstream processing of luciferase from Photinus pyralis is carried out using polymer/salt aqueous two phase system (ATPS) at 4°C. The enzyme is observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) are observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21 | Photinus pyralis |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Photinus pyralis |