Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | - |
Bos taurus |
General Stability | Organism |
---|---|
dialysis, 4°C, 50% loss of activity overnight | Bos taurus |
ultra-filtration, 4°C, 50% loss of activity overnight | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha'-bipyridyl | no activity at 2 mM | Bos taurus | |
Ba2+ | - |
Bos taurus | |
Ca2+ | - |
Bos taurus | |
Cd2+ | - |
Bos taurus | |
Co2+ | - |
Bos taurus | |
Cu2+ | - |
Bos taurus | |
dioxane | - |
Bos taurus | |
Hg2+ | - |
Bos taurus | |
Mg2+ | - |
Bos taurus | |
Mn2+ | - |
Bos taurus | |
Ni2+ | - |
Bos taurus | |
Pb2+ | - |
Bos taurus | |
Sn2+ | - |
Bos taurus | |
Zn2+ | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bos taurus | |
0.012 | - |
Fe2+ | - |
Bos taurus | |
0.51 | - |
gamma-butyrobetaine | - |
Bos taurus | |
0.82 | - |
2-oxoglutarate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Bos taurus |
46000 | - |
2 * 46000, SDS-PAGE | Bos taurus |
80000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | Bos taurus | gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | powder, retains full activity of fresh liver homogenate | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.053 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-90°C, stable for a year or more as acetone powder, stable for 3 months as purified and lyophilized enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | - |
Bos taurus | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? | |
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis | Bos taurus | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Bos taurus |
dimer | 2 * 46000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Bos taurus |