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Literature summary for 1.14.11.16 extracted from

  • Jia, S.; McGinnis, K.; VanDusen, W.J.; Burke, C.J.; Kuo, A.; Griffin, P.R.; Sardana, M.K.; Elliston, K.O.; Stern, A.M.; Friedman, P.A.
    A fully active catalytic domain of bovine aspartyl (asparaginyl) beta-hydroxylase expressed in Escherichia coli: characterization and evidence for the identification of an active-site region in vertebrate alpha-ketoglutarate-dependent dioxygenases (1994), Proc. Natl. Acad. Sci. USA, 91, 7227-7231.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in wild-type Escherichia coli and in Escherichia coli BL21 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, native enzyme Bos taurus
0.024
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, recombinant enzyme Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50700
-
 sedimentation equilibrium Bos taurus
51030
-
 MALDI-TOF Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
first epidermal growth factor-like domain-L-aspartate + 2-oxoglutarate + O2
-
 Bos taurus first epidermal growth factor-like domain-3-hydroxy-L-aspartate + succinate + CO2
-
 r
peptide L-asparagine + 2-oxoglutarate + O2 first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18 Bos taurus peptide 3-hydroxy-L-asparagine + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50700, sedimentation equilibrium Bos taurus