Cloned (Comment) | Organism |
---|---|
expressed in wild-type Escherichia coli and in Escherichia coli BL21 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, native enzyme | Bos taurus | |
0.024 | - |
first growth factor-like domain-L-aspartate | substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, recombinant enzyme | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50700 | - |
sedimentation equilibrium | Bos taurus |
51030 | - |
MALDI-TOF | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
first epidermal growth factor-like domain-L-aspartate + 2-oxoglutarate + O2 | - |
Bos taurus | first epidermal growth factor-like domain-3-hydroxy-L-aspartate + succinate + CO2 | - |
r | |
peptide L-asparagine + 2-oxoglutarate + O2 | first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18 | Bos taurus | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50700, sedimentation equilibrium | Bos taurus |