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Literature summary for 1.14.11.2 extracted from

  • Gunzler, V.; Brocks, D.; Henke, S.; Myllylä, R.; Geiger, R.; Kivirikko, K.I.
    Syncatalytic inactivation of prolyl 4-hydroxylase by synthetic peptides containing the unphysiologic amino acid 5-oxaproline (1988), J. Biol. Chem., 263, 19498-19504.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
bovine serum albumin activation Homo sapiens
catalase activation Gallus gallus
catalase activation Homo sapiens
dithiothreitol activation Gallus gallus
dithiothreitol activation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-Phe-Opr-Gly-benzyl ester 50% inactivation in 1 h at 0.0008 mM, the most effective inhibitor within oxaproline peptides Gallus gallus
benzyloxycarbonyl-Phe-Opr-Gly-benzyl ester 50% inactivation in 1 h at 0.0008 mM, the most effective inhibitor within oxaproline peptides Homo sapiens
additional information synthetic peptides containing the non-physiologic amino acid 5-oxaproline in the sequence R1-Xaa-oxaproline-Gly-OR2 are specific syncatalytic inactivators, noncompetitive inhibition with respect to peptide substrate and ascorbate, compounds with aromatic substituents R1 and R2 are particularly effective when compared with those with an aliphatic group, inactivation is only observed in the presence of Fe2+ and 2-oxoglutarate Gallus gallus
additional information synthetic peptides containing the non-physiologic amino acid 5-oxaproline in the sequence R1-Xaa-oxaproline-Gly-OR2 are specific syncatalytic inactivators, noncompetitive inhibition with respect to peptide substrate and ascorbate, compounds with aromatic substituents R1 and R2 are particularly effective when compared with those with an aliphatic group, inactivation is only observed in the presence of Fe2+ and 2-oxoglutarate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Gallus gallus
additional information
-
additional information
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Gallus gallus
Fe2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Gallus gallus
using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-
placenta
-
Homo sapiens
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus
37
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
Gallus gallus
ascorbate
-
Homo sapiens