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Literature summary for 1.14.11.2 extracted from

  • Nietfeld, J.J.; Kemp, A.
    Properties of prolyl 4-hydroxylase containing firmly-bound iron (1980), Biochim. Biophys. Acta, 613, 349-358.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
(Pro-Pro-Gly)5 at concentrations higher than 0.56 mM, substrate inhibition observed Gallus gallus
EDTA 98-100% inhibition at 0.01 mM Gallus gallus
sodium pyrocatechol disulfonate 98-100% inhibition at 0.1 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
2-oxoglutarate
-
Gallus gallus
0.4 0.5 (L-Pro-L-Pro-Gly)5
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mol/mol, firmly bound, required for catalytic activity, the iron is not part of a 2Fe-2S or a 4Fe-4S cluster Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus
62000
-
2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay method Gallus gallus
additional information
-
enzyme activity after dialysis against iron chelators Gallus gallus
0.13
-
reaction mixture lacking catalase Gallus gallus
0.2
-
reaction mixture lacking serum albumin Gallus gallus
0.6
-
reaction mixture lacking dithiothreitol Gallus gallus
0.75
-
reaction mixture lacking Fe2+ Gallus gallus
1.8 2.6
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
Gallus gallus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
r
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 58000 + 2 * 62000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ascorbate completely dependent, required for activity Gallus gallus