Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(Pro-Pro-Gly)5 | at concentrations higher than 0.56 mM, substrate inhibition observed | Gallus gallus | |
EDTA | 98-100% inhibition at 0.01 mM | Gallus gallus | |
sodium pyrocatechol disulfonate | 98-100% inhibition at 0.1 mM | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
2-oxoglutarate | - |
Gallus gallus | |
0.4 | 0.5 | (L-Pro-L-Pro-Gly)5 | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mol/mol, firmly bound, required for catalytic activity, the iron is not part of a 2Fe-2S or a 4Fe-4S cluster | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000 + 2 * 62000, SDS-PAGE | Gallus gallus |
62000 | - |
2 * 58000 + 2 * 62000, SDS-PAGE | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Gallus gallus |
additional information | - |
enzyme activity after dialysis against iron chelators | Gallus gallus |
0.13 | - |
reaction mixture lacking catalase | Gallus gallus |
0.2 | - |
reaction mixture lacking serum albumin | Gallus gallus |
0.6 | - |
reaction mixture lacking dithiothreitol | Gallus gallus |
0.75 | - |
reaction mixture lacking Fe2+ | Gallus gallus |
1.8 | 2.6 | - |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2 | - |
Gallus gallus | (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 58000 + 2 * 62000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | completely dependent, required for activity | Gallus gallus |