Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxosuccinate | i.e. oxaloacetate | Gallus gallus | |
3-hydroxybutyrate | less than 10% inhibition | Gallus gallus | |
ascorbate | at high concentrations | Gallus gallus | |
citrate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
epinephrine | competitive inhibition with respect to Fe2+ | Gallus gallus | |
Fe2+ | inhibitory at high concentrations | Gallus gallus | |
fumarate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
isocitrate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
Lactate | less than 10% inhibition | Gallus gallus | |
malate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
nitroblue tetrazolium | competitive inhibition with respect to O2 | Gallus gallus | |
oxaloacetate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
poly(L-proline) | - |
Gallus gallus | |
pyruvate | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
Zn2+ | competitive inhibition with respect to Fe2+, non-competitive with respect to the polypeptide substrate and 2-oxoglutarate | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | absolute requirement, Km: 0.005 mM | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 | mechanism, role of cosubstrates | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
2-oxoglutarate + O2 + ascorbate | uncoupled oxidative decarboxylation | Gallus gallus | succinate + dehydroascorbate + CO2 + H2O | - |
? | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Gallus gallus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | Km: 0.2 mM | Gallus gallus | |
ascorbate | requirement | Gallus gallus | |
additional information | the ascorbate requirement cannot be replaced by tetrahydrofolic acid, dithiothreitol, NADH2 or dithionite to any significant extent | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
Zn2+ | - |
Gallus gallus | |
0.04 | - |
nitroblue tetrazolium | - |
Gallus gallus | |
0.1 | - |
epinephrine | - |
Gallus gallus | |
0.4 | 0.5 | citrate | calculated from the slopes or intercepts | Gallus gallus | |
0.5 | 0.6 | fumarate | calculated from the slopes or intercepts | Gallus gallus | |
0.6 | - |
Fe2+ | - |
Gallus gallus | |
0.6 | - |
oxaloacetate | - |
Gallus gallus | |
1.2 | 1.3 | malate | calculated from the slopes or intercepts | Gallus gallus | |
1.5 | 2.1 | isocitrate | calculated from the slopes or intercepts | Gallus gallus | |
7 | - |
ascorbate | - |
Gallus gallus | |
15 | 18 | pyruvate | calculated from the slopes or intercepts | Gallus gallus |