Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO2 | 5% inhibition at 3.6 mM, 35% inhibition at 7.2 mM and 75% inhibition at 12 mM | Gallus gallus | |
Collagen | product inhibitor, noncompetitive with respect to all substrates of the reaction | Gallus gallus | |
poly(L-proline) | competitive inhibitor with respect to the polypeptide substrate and uncompetitive with respect to Fe2+ and 2-oxoglutarate | Gallus gallus | |
succinate | competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 | mechanism, kinetic analysis of the reaction sequence | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | 1.6 | succinate | calculated from the slopes or intercepts | Gallus gallus | |
2.3 | 2.5 | poly(L-proline) | calculated from the slopes or intercepts | Gallus gallus | |
40 | - |
Collagen | from basement membranes, calculated from the slopes or intercepts | Gallus gallus | |
170 | 180 | Collagen | from skin, calculated from the slopes or intercepts | Gallus gallus |