Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Catharanthus roseus | |
dithiothreitol | activation | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
potassium phosphate | - |
Catharanthus roseus | |
Sodium acetate | - |
Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
O2 | - |
Catharanthus roseus | |
0.23 | - |
poly(L-proline) | - |
Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | 30% of the enzyme activity | Lolium multiflorum | 5783 | - |
Golgi apparatus | 70% of the enzyme activity | Lolium multiflorum | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Daucus carota | |
Fe2+ | - |
Catharanthus roseus | |
Fe2+ | - |
Lolium multiflorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Daucus carota | - |
- |
- |
Lolium multiflorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using ammonium sulfate precipitation, column chromatography on DEAE-Sephadex A-50 and affinity chromatography on agarose-linked poly(L-proline) column | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | - |
Catharanthus roseus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | - |
? | |
Gly-Pro8 + 2-oxoglutarate + O2 | 96% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? | |
additional information | no reaction with: free proline, prolyl peptides whose residues number is four or less | Daucus carota | ? | - |
? | |
additional information | no reaction with: free proline, prolyl peptides whose residues number is four or less | Catharanthus roseus | ? | - |
? | |
additional information | no reaction with: free proline, prolyl peptides whose residues number is four or less | Lolium multiflorum | ? | - |
? | |
octa-L-proline + 2-oxoglutarate + O2 | 81.8% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | - |
Lolium multiflorum | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | MW 2000, 6000 and 12000 | Catharanthus roseus | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-proline) + 2-oxoglutarate + O2 | - |
Catharanthus roseus | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
r | |
protocollagen + 2-oxoglutarate + O2 | - |
Daucus carota | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Catharanthus roseus | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Lolium multiflorum | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 | 91.4% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Catharanthus roseus |
7.5 | - |
- |
Lolium multiflorum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | Km: 0.09 mM | Catharanthus roseus | |
ascorbate | - |
Daucus carota | |
ascorbate | - |
Catharanthus roseus | |
ascorbate | - |
Lolium multiflorum |