Literature summary for 1.14.11.2 extracted from

Kaska, D.D.; Myllyl�, R.; Gunzler, V.; Gibori, A.; Kivirikko, K.I.
Prolyl 4-hydroxylase from Volvox carteri. A low-Mr enzyme antigenically related to the alpha subunit of the vertebrate enzyme (1988), Biochem. J., 256, 257-263.

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Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-Dihydroxyphenylacetate	-	Helianthus tuberosus
3,4-Dihydroxyphenylacetate	-	Persea americana
3,4-Dihydroxyphenylacetate	-	Ulva intestinalis
3,4-Dihydroxyphenylacetate	-	Volvox carteri
3,4-Dihydroxyphenylpropionate	-	Helianthus tuberosus
3,4-Dihydroxyphenylpropionate	-	Persea americana
3,4-Dihydroxyphenylpropionate	-	Ulva intestinalis
3,4-Dihydroxyphenylpropionate	-	Volvox carteri
Pyridine 2,4-dicarboxylate	-	Volvox carteri
Pyridine 2,5-dicarboxylate	-	Volvox carteri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	poly(L-proline)	-	Volvox carteri
0.17	-	2-oxoglutarate	-	Volvox carteri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	-	Helianthus tuberosus
Fe2+	-	Persea americana
Fe2+	-	Ulva intestinalis
Fe2+	Km: 0.02 mM	Volvox carteri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	gel filtration	Ulva intestinalis
40000	-	gel filtration	Volvox carteri
65000	-	1 * 65000, SDS-PAGE	Volvox carteri

Organism

Organism	UniProt	Comment	Textmining
Helianthus tuberosus	-	-	-
Persea americana	-	-	-
Ulva intestinalis	-	-	-
Volvox carteri	-	green algae	-

Purification (Commentary)

Purification (Comment)	Organism
using ion-exchange chromatography on DEAE-cellulose, and affinity chromatography on poly(L-hydroxyproline), 3,4-dihydroxyphenylacetate or 3,4-dihydroxyphenylpropionate linked to Sepharose	Volvox carteri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Helianthus tuberosus
additional information	-	-	Persea americana
additional information	-	-	Ulva intestinalis
additional information	-	-	Volvox carteri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	proline or the dipeptides Ser-Pro or Ala-Pro do not serve as substrates	Volvox carteri	?	-	?
poly(L-Pro) + 2-oxoglutarate + O2	-	Helianthus tuberosus	poly(4-hydroxyproline) + succinate + CO2	-	?
poly(L-Pro) + 2-oxoglutarate + O2	-	Persea americana	poly(4-hydroxyproline) + succinate + CO2	-	?
poly(L-Pro) + 2-oxoglutarate + O2	-	Ulva intestinalis	poly(4-hydroxyproline) + succinate + CO2	-	?
poly(L-Pro) + 2-oxoglutarate + O2	-	Volvox carteri	poly(4-hydroxyproline) + succinate + CO2	-	?
poly(L-proline) + 2-oxoglutarate + O2	-	Volvox carteri	poly(trans-4-hydroxy-L-proline) + succinate + CO2	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 65000, SDS-PAGE	Volvox carteri

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Volvox carteri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	6.8	-	Volvox carteri

Cofactor

Cofactor	Comment	Organism	Structure
ascorbate	-	Helianthus tuberosus
ascorbate	-	Persea americana
ascorbate	-	Ulva intestinalis
ascorbate	Km: 0.14 mM	Volvox carteri

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.008	-	Pyridine 2,4-dicarboxylate	-	Volvox carteri
0.02	-	Pyridine 2,5-dicarboxylate	-	Volvox carteri
0.024	-	3,4-Dihydroxyphenylpropionate	-	Volvox carteri
0.04	-	3,4-Dihydroxyphenylacetate	-	Volvox carteri
3	-	poly(L-hydroxyproline)	-	Volvox carteri

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Release 2023.1 (January 2023)