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Literature summary for 1.14.11.2 extracted from

  • Koivu, J.; Myllylä, R.
    Protein disulfide-isomerase retains procollagen prolyl 4-hydroxylase structure in its native conformation (1986), Biochemistry, 25, 5982-5986.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
additional information protein disulfide-isomerase is able to activate the purified enzyme, the activation is probably due to the repairing of disulfide exchanges ocurring in the prolyl 4-hydroxylase structure during purification and storage. Protein disulfide-isomerase is also able to reactivate the enzyme inactivated by mild H2O2 treatment Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 95-100% inhibition at 0.45 mM Gallus gallus
H2O2 94% inhibition at 1 M, dissociation of the enzyme, 12% of the enzyme remains in the tetrameric form Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Gallus gallus
-
-

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
protein disulfide-isomerase may be an enzyme that is able to retain the prolyl 4-hydroxylase structure in its native conformation, but it seems to lack the ability to associate the enzyme monomers into the active tetrameric form Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity before and after treatment with protein disulfide-isomerase Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, in microsomes, less than 10% inactivation, 10 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protocollagen + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?