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Literature summary for 1.14.11.2 extracted from
- The Skp1 prolyl hydroxylase from Dictyostelium is related to the hypoxia-inducible factor-alpha class of animal prolyl 4-hydroxylases (2005), J. Biol. Chem., 280, 14645-14655.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Dictyostelium discoideum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dihydroxybenzoate | - |
Dictyostelium discoideum |  |
| 3,4-dihydroxyphenyl acetate | IC50: 0.3 mM | Dictyostelium discoideum | |
| CoCl2 | IC50: 0.00025 mM | Dictyostelium discoideum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
His10-rSkp1A | - |
Dictyostelium discoideum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Dictyostelium discoideum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Dictyostelium discoideum |
| 33350 | - |
calculated from sequence of cDNA | Dictyostelium discoideum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | Q86KR9 | - |
- |
| Dictyostelium discoideum AX3 | Q86KR9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose fast flow anion exchange chromatography, phenyl-Sepharose fast flow column chromatography and Source 15Q anion exchange chromatography | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| His10-rSkp1A + ? | - |
Dictyostelium discoideum | ? | - |
? | |
| His10-rSkp1A + ? | - |
Dictyostelium discoideum AX3 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hypoxia-inducible factor -1alpha-type prolyl 4-hydroxylase | - |
Dictyostelium discoideum |
| P4H1 | - |
Dictyostelium discoideum |
| prolyl-4-hydroxylase | - |
Dictyostelium discoideum |
| Skp1 prolyl hydroxylase | - |
Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | dependent on physiological concentrations of ascorbate | Dictyostelium discoideum | |
| O2 | dependent on physiological concentrations of O2 | Dictyostelium discoideum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Dictyostelium discoideum | predicted value according to the amino acid sequence | - |
6.9 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
IC50: 0.00025 mM | Dictyostelium discoideum | CoCl2 | |
| 0.3 | - |
IC50: 0.3 mM | Dictyostelium discoideum | 3,4-dihydroxyphenyl acetate | |
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