Cloned (Comment) | Organism |
---|---|
isozymes CS1 and CS2, overexpression in Escherichia coli JM101 | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | competitive | Streptomyces clavuligerus | |
N-bromoacetyl-L-arginine | isozyme CS2, inactivation, at 7.5 mM half-life of 11.2 min at 30°C, Co2+ competes, substrates protect | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Streptomyces clavuligerus | |
Fe2+ | 1 ion per catalytically active enzyme molecule for the cyclization and the saturation reaction | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | this reaction is due to a proclavaminate amidinohydrolase, i.e. cla | amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | Streptomyces clavuligerus | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | ? | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
dihydroclavaminate + succinate + CO2 + H2O | product is a transient intermediate in the overall reaction process | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
isozyme CS2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 | this reaction is due to a proclavaminate amidinohydrolase PAH, gene cla | Streptomyces clavuligerus | |
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | this reaction is due to a proclavaminate amidinohydrolase, i.e. cla | Streptomyces clavuligerus | amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | dependent on O2 and 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
additional information | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | Streptomyces clavuligerus | ? | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | isozyme CS2 | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | product is a transient intermediate in the overall reaction process | ? | |
proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | product is a transient intermediate in the overall reaction process | ? |
Synonyms | Comment | Organism |
---|---|---|
CS | - |
Streptomyces clavuligerus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
38.3 | - |
N-bromoacetyl-L-arginine | isozyme CS2, pH 7.0, 30°C | Streptomyces clavuligerus |