Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium chloride | - |
Acremonium chrysogenum | |
ammonium sulfate | - |
Acremonium chrysogenum | |
ascorbate | required | Acremonium chrysogenum | |
dithiothreitol | up to 30% activation in presence of dithiothreitol | Acremonium chrysogenum |
General Stability | Organism |
---|---|
sodium phosphate and Bis/Tris buffers are inhibitory | Acremonium chrysogenum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate | reversible, 0.04 mM, 90% inhibition | Acremonium chrysogenum | |
ammonium hydrogencarbonate | 100-500 mM, complete inactivation | Acremonium chrysogenum | |
bathophenanthroline | - |
Acremonium chrysogenum | |
N-ethylmaleimide | - |
Acremonium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Acremonium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
Purification (Comment) | Organism |
---|---|
- |
Acremonium chrysogenum |
Storage Stability | Organism |
---|---|
-70°C, presence of dithiothreitol, stable for 4 weeks | Acremonium chrysogenum |
4°C, presence of dithiothreitol, half-life is 48 h | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Acremonium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acremonium chrysogenum |