Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
Cu2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
EDTA | more than 50% inhibition at 1 mM | Streptomyces sp. | |
Ni2+ | more than 50% inhibition at 1 mM | Streptomyces sp. | |
Zn2+ | more than 50% inhibition at 1 mM | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
2-oxoglutarate | - |
Streptomyces sp. | |
0.083 | - |
2-oxoglutarate | - |
Streptomyces sp. | |
0.2 | - |
L-proline | - |
Streptomyces sp. | |
0.43 | - |
L-proline | - |
Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | strictly required | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33150 | - |
calculated | Streptomyces sp. |
33570 | - |
calculated | Streptomyces sp. |
35000 | - |
apparent molecular weight on SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
strainTH1 | - |
Streptomyces sp. TH1 | - |
strainTH1 | - |
Purification (Comment) | Organism |
---|---|
2 chromatographic steps to homogeneity | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces sp. |
12.7 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dehydro-L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3,4-epoxy-L-proline + succinate + CO2 | - |
? | |
3,4-dehydro-L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3,4-epoxy-L-proline + succinate + CO2 | - |
? | |
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2 | - |
? | |
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2 | - |
? | |
L-pipecolic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-pipecolic acid + succinate + CO2 | - |
? | |
L-pipecolic acid + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-pipecolic acid + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
proline 3-hydroxylase type I | - |
Streptomyces sp. |
proline 3-hydroxylase type II | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces sp. |
40 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
below 30°C activity remained above 85% after 30 min at pH 6.0 | Streptomyces sp. |
40 | - |
below 40°C activity remained above 85% after 30 min at pH 6.0 | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | activity remained above 85% after 16 h at 4°C | Streptomyces sp. |
5.5 | 8.5 | activity remained above 85% after 16 h at 4°C | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | strictly required | Streptomyces sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. | - |
- |
4.3 |
Streptomyces sp. | - |
- |
4.6 |