Cloned (Comment) | Organism |
---|---|
phylogenetic tree, T6ODM real time quantitative PCR expression analysis | Papaver somniferum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thebaine + 2-oxoglutarate + O2 | Papaver somniferum | - |
neopinone + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | - |
cv. Bea's Choice | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-canadine + 2-oxoglutarate + O2 | a protoberberine, 32% of activity with oripavine | Papaver somniferum | ? | - |
? | |
(R,S)-tetrahydropalmatine + 2-oxoglutarate + O2 | a protoberberine, O-demethylation, below 1% of activity with oripavine | Papaver somniferum | ? | - |
? | |
(R,S)-tetrahydropapaverine + 2-oxoglutarate + O2 | a 1-benzylisoquinoline, O-demethylation, 2.1% of activity with oripavine | Papaver somniferum | ? | - |
? | |
allocryptopine + 2-oxoglutarate + O2 | a protopine, O-demethylation, 9.5% of activity with oripavine | Papaver somniferum | ? | - |
? | |
cryptopine + 2-oxoglutarate + O2 | a protopine, O-demethylation, below 1% of activity with oripavine | Papaver somniferum | ? | - |
? | |
additional information | when assayed with a wide range of benzylisoquinoline alkaloids, the enzyme shows efficient dealkylation activities, including regio- and substrate-specific O-demethylation and O,O-demethylenation, substrate specificity, overview. No or poor activity with (S)-reticuline, (S)-isocorydine, (S)-scoulerine, (R,S)-stylopine, berberine, O-demethylcryptopine, protopine, and codeine | Papaver somniferum | ? | - |
? | |
oripavine + 2-oxoglutarate + O2 | a morphinan, best substrate | Papaver somniferum | ? | - |
? | |
papaverine + 2-oxoglutarate + O2 | a 1-benzylisoquinoline,O-demethylation, below 1% of activity with oripavine | Papaver somniferum | ? | - |
? | |
thebaine + 2-oxoglutarate + O2 | - |
Papaver somniferum | neopinone + formaldehyde + succinate + CO2 | - |
? | |
thebaine + 2-oxoglutarate + O2 | a morphinan, 97.2% of activity with oripavine | Papaver somniferum | neopinone + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T6ODM | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate/Fe(II)-dependent dioxygenase superfamily | Papaver somniferum |
metabolism | thebaine 6-O-demethylase catalyzes the antepenultimate step of morphine biosynthesis in opium poppy | Papaver somniferum |
physiological function | thebaine 6-O-demethylase catalyzes the antepenultimate step of morphine biosynthesis in opium poppy, but has also roles in the metabolism of protopine, benzo[c]phenanthridine, and rhoeadine alkaloids | Papaver somniferum |