Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
AlkB in complex with double-stranded DNA containing 1,N6-ethenoadenine, 3-methylthymine, and 3-methylcytosine, using 100 mM NaCl, 25 mM MgCl2, 100 mM cacodylate (pH 6.5) and 20-24% (w/v) PEG 8K or 4K | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D135A | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
D135N | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
D135S | the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05050 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono-Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1,N6-ethenoadenine + 2-oxoglutarate + O2 | - |
Escherichia coli | ? | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | preferred substrate of AlkB | Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylthymine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-thymine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli |