Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, and functional expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3) | Acanthamoeba polyphaga Mimivirus |
Protein Variants | Comment | Organism |
---|---|---|
D250A | site-directed mutagenesis, the mutant shows unaltered lysyl hydroxylase activity, although reduced collagen glucosyltransferase activity, compared to the wild-type enzyme | Acanthamoeba polyphaga Mimivirus |
D97A/D99A | site-directed mutagenesis, the mutant cannot be expressed in Escherichia coli | Acanthamoeba polyphaga Mimivirus |
H80A | site-directed mutagenes, the mutant cannot be expressed in Escherichia coli | Acanthamoeba polyphaga Mimivirus |
H825S/D827A | site-directed mutagenesis, lysyl hydroxylase inactive mutant, that is still active as collagen glucosyltransferase | Acanthamoeba polyphaga Mimivirus |
L78K | site-directed mutagenesis, the mutant cannot be expressed in Escherichia coli | Acanthamoeba polyphaga Mimivirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Acanthamoeba polyphaga Mimivirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine-[procollagen] + 2-oxoglutarate + O2 | Acanthamoeba polyphaga Mimivirus | - |
(2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2 | - |
? | |
L-lysine-[procollagen] + 2-oxoglutarate + O2 | Acanthamoeba polyphaga Mimivirus L230 | - |
(2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2 | - |
? | |
additional information | Acanthamoeba polyphaga Mimivirus | bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen | ? | - |
? | |
additional information | Acanthamoeba polyphaga Mimivirus L230 | bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba polyphaga Mimivirus | - |
- |
- |
Acanthamoeba polyphaga Mimivirus L230 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Acanthamoeba polyphaga Mimivirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GDK)4 + 2-oxoglutarate + O2 | peptide acceptor substrate | Acanthamoeba polyphaga Mimivirus | ? | - |
? | |
(GDK)4 + 2-oxoglutarate + O2 | peptide acceptor substrate | Acanthamoeba polyphaga Mimivirus L230 | ? | - |
? | |
L-lysine-[procollagen] + 2-oxoglutarate + O2 | - |
Acanthamoeba polyphaga Mimivirus | (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2 | - |
? | |
L-lysine-[procollagen] + 2-oxoglutarate + O2 | - |
Acanthamoeba polyphaga Mimivirus L230 | (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2 | - |
? | |
additional information | bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen | Acanthamoeba polyphaga Mimivirus | ? | - |
? | |
additional information | the enzyme sequentially hydroxylates and glucosylates collagensubstrates in vitro | Acanthamoeba polyphaga Mimivirus | ? | - |
? | |
additional information | bifunctional lysyl hydroxylase and glycosyltransferase enzyme, mimivirus L230 is capable of hydroxylating lysine and glycosylating the resulting hydroxylysine residues in a native mimivirus collagen acceptor substrate. In contrast to other animals, the mimivirus L230 enzyme transfers glucose instead of galactose to hydroxylysine in collagen | Acanthamoeba polyphaga Mimivirus L230 | ? | - |
? | |
additional information | the enzyme sequentially hydroxylates and glucosylates collagensubstrates in vitro | Acanthamoeba polyphaga Mimivirus L230 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L230 | - |
Acanthamoeba polyphaga Mimivirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
General Information | Comment | Organism |
---|---|---|
evolution | whereas in animals from sponges to humans the transfer of galactose to hydroxylysine in collagen is conserved, the mimivirus L230 enzyme transfers glucose to hydroxylysine, thereby defining another type of collagen glycosylation in nature. Utilization of glucose instead of the galactose found throughout animals as well as a bifunctional enzyme rather than two separate enzymes may represent a parallel evolutionary track in collagen biology | Acanthamoeba polyphaga Mimivirus |