Cloned (Comment) | Organism |
---|---|
gene Plod3, expression of recombinant V5/His-tagged LH3 protein in HEK-293 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of mouse osteoblastic cell line, MC3T3-E1, stably suppressing Plod3 expresion using short hairpin RNA technology, reduced LH3 protein levels in the Sh clones, phenotype, overview | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen | ? | - |
? | |
additional information | Mus musculus CRL-2593 | lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9R0E1 | gene Plod3 | - |
Mus musculus CRL-2593 | Q9R0E1 | gene Plod3 | - |
Purification (Comment) | Organism |
---|---|
recombinant V5/His-tagged LH3 protein from HEK-293 cells by nickel affinity chromatography, dialysis, and ultrafiltration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MC3T3-E1 cell | - |
Mus musculus | - |
osteoblast | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen | Mus musculus | ? | - |
? | |
additional information | lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen | Mus musculus CRL-2593 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LH | - |
Mus musculus |
lysyl hydroxylase 3 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | impairment of LH3 function significantly affects type I collagen fibrillogenesis | Mus musculus |
physiological function | lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture, role of LH3 in bone physiology | Mus musculus |