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Literature summary for 1.14.11.6 extracted from

  • Vainio, S.; Genest, P.A.; ter Riet, B.; van Luenen, H.; Borst, P.
    Evidence that J-binding protein 2 is a thymidine hydroxylase catalyzing the first step in the biosynthesis of DNA base J (2009), Mol. Biochem. Parasitol., 164, 157-161.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues Leishmania tarentolae

Protein Variants

Protein Variants Comment Organism
D416A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect Leishmania tarentolae
H414A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect Leishmania tarentolae
H465A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect Leishmania tarentolae
additional information inactivation of both of the LtJBP2 alleles by conventional knock-out strategy, the major phenotypes of the LtJBP2-/- cells, showing reduced levels of J and serious growth defects, can be completely reversed by the presence of an ectopic copy of LtJBP2 Leishmania tarentolae
R478A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect Leishmania tarentolae
V482A/L548Q site-directed and random mutagenesis, the mutant can complement the JBP2 knockout mutant strain Leishmania tarentolae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymine + 2-oxoglutarate + O2 Leishmania tarentolae
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5-hydroxymethyluracil + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Leishmania tarentolae B6EU02
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a bifunctional helicase and thymine dioxygenase Leishmania tarentolae ?
-
?
thymine + 2-oxoglutarate + O2
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Leishmania tarentolae 5-hydroxymethyluracil + succinate + CO2
-
?

Subunits

Subunits Comment Organism
More JBP2 contains a SWI2/SNF2 chromatin remodeling domain in its C-terminus, and a homologous region with JBP1 at the N-terminus Leishmania tarentolae

Synonyms

Synonyms Comment Organism
J-binding protein 2
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Leishmania tarentolae
JBP2
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Leishmania tarentolae
More the enzyme belongs to the the family of Fe(II)-2OG-dependent dioxygenases Leishmania tarentolae
thymidine hydroxylase
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Leishmania tarentolae

General Information

General Information Comment Organism
physiological function the genomic DNA of kinetoplastid parasites contains a unique modified base beta-D-glucosylhydroxymethyluracil or base J. J-binding protein, JBP, 1 and 2, which regulate the levels of J in the genome, are catalyzing the first step in J biosynthesis. JBP2, like JBP1, is a thymidine hydroxylase. Unlike JBP1, JBP2 is dispensable for Leishmania, mutational analysis, overview Leishmania tarentolae