Cloned (Comment) | Organism |
---|---|
JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues | Leishmania tarentolae |
Protein Variants | Comment | Organism |
---|---|---|
D416A | site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect | Leishmania tarentolae |
H414A | site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect | Leishmania tarentolae |
H465A | site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect | Leishmania tarentolae |
additional information | inactivation of both of the LtJBP2 alleles by conventional knock-out strategy, the major phenotypes of the LtJBP2-/- cells, showing reduced levels of J and serious growth defects, can be completely reversed by the presence of an ectopic copy of LtJBP2 | Leishmania tarentolae |
R478A | site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect | Leishmania tarentolae |
V482A/L548Q | site-directed and random mutagenesis, the mutant can complement the JBP2 knockout mutant strain | Leishmania tarentolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + 2-oxoglutarate + O2 | Leishmania tarentolae | - |
5-hydroxymethyluracil + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | B6EU02 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a bifunctional helicase and thymine dioxygenase | Leishmania tarentolae | ? | - |
? | |
thymine + 2-oxoglutarate + O2 | - |
Leishmania tarentolae | 5-hydroxymethyluracil + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | JBP2 contains a SWI2/SNF2 chromatin remodeling domain in its C-terminus, and a homologous region with JBP1 at the N-terminus | Leishmania tarentolae |
Synonyms | Comment | Organism |
---|---|---|
J-binding protein 2 | - |
Leishmania tarentolae |
JBP2 | - |
Leishmania tarentolae |
More | the enzyme belongs to the the family of Fe(II)-2OG-dependent dioxygenases | Leishmania tarentolae |
thymidine hydroxylase | - |
Leishmania tarentolae |
General Information | Comment | Organism |
---|---|---|
physiological function | the genomic DNA of kinetoplastid parasites contains a unique modified base beta-D-glucosylhydroxymethyluracil or base J. J-binding protein, JBP, 1 and 2, which regulate the levels of J in the genome, are catalyzing the first step in J biosynthesis. JBP2, like JBP1, is a thymidine hydroxylase. Unlike JBP1, JBP2 is dispensable for Leishmania, mutational analysis, overview | Leishmania tarentolae |