Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.9 extracted from

  • Flachowsky, H.; Halbwirth, H.; Treutter, D.; Richter, K.; Hanke, M.V.; Szankowski, I.; Gosch, C.; Stich, K.; Fischer, T.C.
    Silencing of flavanone-3-hydroxylase in apple (Malus x domestica Borkh.) leads to accumulation of flavanones, but not to reduced fire blight susceptibility (2012), Plant Physiol. Biochem., 51, 18-25.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene fht, transcription profiling and quantitative real-time PCR expression analysis Malus domestica

Protein Variants

Protein Variants Comment Organism
additional information F3H silencing by fht antisense transgenic apple clones construction, diverse transgenic lines, phenotypes, overview Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
prohexadione-Ca an enzyme inhibitor for 2-oxoglutarate dependent dioxygenases, used as a growth retardant and for control of secondary fire blight, Erwinia amylovora, of apple leaves Malus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a flavanone + 2-oxoglutarate + O2 Malus domestica
-
a dihydroflavonol + succinate + CO2
-
?
eriodyctiol + 2-oxoglutarate + O2 Malus domestica
-
dihydroquercetin + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Malus domestica
-
cv. Pinova, gene fht
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a flavanone + 2-oxoglutarate + O2
-
Malus domestica a dihydroflavonol + succinate + CO2
-
?
eriodyctiol + 2-oxoglutarate + O2
-
Malus domestica dihydroquercetin + succinate + CO2
-
?
naringenin + 2-oxoglutarate + O2
-
Malus domestica dihydrokaempferol + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
F3H
-
Malus domestica
FHT
-
Malus domestica
flavanone-3-hydroxylase
-
Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.25
-
assay at Malus domestica

Expression

Organism Comment Expression
Malus domestica downregulation of flavanone 4-reductase leads to upregulation of other flavanoid pathway genes, inclunding the flavanone 3-hydroxylase, feedback regulation of flavonoid gene expression, overview up

General Information

General Information Comment Organism
evolution the enzyme belongs to the 2-oxoglutarate-dependant dioxygenases Malus domestica
malfunction silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-deoxyflavonoid luteoforol is formed from accumulating flavanones, acting as an antimicrobial compound against the fire blight pathogen Erwinia amylovora. Inducible resistance to fire blight by prohexadione-Ca is not observed with the antisense flavanone-3-hydroxylase apple plants Malus domestica