Cloned (Comment) | Organism |
---|---|
gene fht, transcription profiling and quantitative real-time PCR expression analysis | Malus domestica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | F3H silencing by fht antisense transgenic apple clones construction, diverse transgenic lines, phenotypes, overview | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
prohexadione-Ca | an enzyme inhibitor for 2-oxoglutarate dependent dioxygenases, used as a growth retardant and for control of secondary fire blight, Erwinia amylovora, of apple leaves | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a flavanone + 2-oxoglutarate + O2 | Malus domestica | - |
a dihydroflavonol + succinate + CO2 | - |
? | |
eriodyctiol + 2-oxoglutarate + O2 | Malus domestica | - |
dihydroquercetin + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cv. Pinova, gene fht | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a flavanone + 2-oxoglutarate + O2 | - |
Malus domestica | a dihydroflavonol + succinate + CO2 | - |
? | |
eriodyctiol + 2-oxoglutarate + O2 | - |
Malus domestica | dihydroquercetin + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Malus domestica | dihydrokaempferol + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3H | - |
Malus domestica |
FHT | - |
Malus domestica |
flavanone-3-hydroxylase | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
assay at | Malus domestica |
Organism | Comment | Expression |
---|---|---|
Malus domestica | downregulation of flavanone 4-reductase leads to upregulation of other flavanoid pathway genes, inclunding the flavanone 3-hydroxylase, feedback regulation of flavonoid gene expression, overview | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate-dependant dioxygenases | Malus domestica |
malfunction | silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-deoxyflavonoid luteoforol is formed from accumulating flavanones, acting as an antimicrobial compound against the fire blight pathogen Erwinia amylovora. Inducible resistance to fire blight by prohexadione-Ca is not observed with the antisense flavanone-3-hydroxylase apple plants | Malus domestica |