Literature summary for 1.14.12.1 extracted from

Beharry, Z.M.; Eby, D.M.; Coulter, E.D.; Viswanathan, R.; Neidle, E.L.; Phillips, R.S.; Kurtz, D.M., Jr.
Histidine ligand protonation and redox potential in the Rieske dioxygenases: Role of a conserved aspartate in anthranilate 1,2-Dioxygenase (2003), Biochemistry, 42, 13625-13636.

Search for more literature for 1.14.12.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes in Escherichia coli BL21-Codon Plus	Acinetobacter sp.

Protein Variants

Protein Variants	Comment	Organism
D218A	no activity	Acinetobacter sp.
D218E	no activity	Acinetobacter sp.
D218N	no activity	Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration	Acinetobacter sp.
52000	-	apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration	Acinetobacter sp.
220000	-	gel filtration, wild type and mutant enzymes	Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
anthranilic acid + NAD(P)H + O2 + H2O	Acinetobacter sp.	initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source	catechol + CO2 + NAD(P)+ + NH3	-	?
anthranilic acid + NAD(P)H + O2 + H2O	Acinetobacter sp. ADP1	initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source	catechol + CO2 + NAD(P)+ + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	-	-	-
Acinetobacter sp. ADP1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild type and mutant enzymes	Acinetobacter sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
anthranilic acid + NAD(P)H + O2 + H2O	initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source	Acinetobacter sp.	catechol + CO2 + NAD(P)+ + NH3	-	?
anthranilic acid + NAD(P)H + O2 + H2O	initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source	Acinetobacter sp. ADP1	catechol + CO2 + NAD(P)+ + NH3	-	?
anthranilic acid + NADH + O2 + H2O	-	Acinetobacter sp.	catechol + CO2 + NAD+ + NH3	-	?
anthranilic acid + NADH + O2 + H2O	-	Acinetobacter sp. ADP1	catechol + CO2 + NAD+ + NH3	-	?

Subunits

Subunits	Comment	Organism
hexamer	apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration	Acinetobacter sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	oxygenase component completely inactive at pH 9	Acinetobacter sp.

Cofactor

Cofactor	Comment	Organism	Structure
iron-sulfur centre	proximal Rieske [2Fe-2S] center	Acinetobacter sp.
NADH	-	Acinetobacter sp.
NADPH	-	Acinetobacter sp.

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Release 2023.1 (January 2023)