Cloned (Comment) | Organism |
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expression of genes encoding toluene dioxygenase complex todC1C2BA in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of a plasmid containing the entire tod operon todC1C2BADE to Pseudomonas putida T57 to enhance its ability to degrade 4-chloroaniline. The resulting strain shows 250-fold higher 4-chloroaniline degradation activity the parental strain. The recobinant strain degrades 2-chloroaniline, 3-chloroaniline, and 3,4-dichloroaniline as well as 3-chloroaniline, but not 3,5-dichloroaniline | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
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Pseudomonas putida | - |
- |
- |
Pseudomonas putida T57 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida | ? + NAD+ | - |
? | |
2-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida T57 | ? + NAD+ | - |
? | |
3,4-dichloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida | ? + NAD+ | - |
? | |
3,4-dichloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida T57 | ? + NAD+ | - |
? | |
3-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida | ? + NAD+ | - |
? | |
3-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida T57 | ? + NAD+ | - |
? | |
4-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida | 4-chlorocatechol + 2-amino-5-chlorophenol + NAD+ | toluene dioxygenase catalyzes both 1,2- and 2,3-dioxygenation of 4-chloroaniline | ? | |
4-chloroaniline + NADH + H+ + O2 | degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline | Pseudomonas putida T57 | 4-chlorocatechol + 2-amino-5-chlorophenol + NAD+ | toluene dioxygenase catalyzes both 1,2- and 2,3-dioxygenation of 4-chloroaniline | ? | |
additional information | no substrate: 3,5-dichloroaniline | Pseudomonas putida | ? | - |
? | |
additional information | no substrate: 3,5-dichloroaniline | Pseudomonas putida T57 | ? | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | expression of genes encoding toluene dioxygenase complex todC1C2BA in Escherichia coli results in the ability to degrade 4-chloroaniline | Pseudomonas putida |