General Stability | Organism |
---|---|
10% ethanol and 10% glycerol are required for stability | Pseudomonas sp. |
DTT stabilizes | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Pseudomonas sp. | |
additional information | no significant activation by the addition of Fe2+, Fe3+, Zn2+, Mg2+ or Cu2+ | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthalene + NADH + O2 | Pseudomonas sp. | initial oxidative reaction in bacterial naphthalene catabolism | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography separates the reductase from the two other enzyme components | Pseudomonas sp. |
purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthalene + NAD(P)H + O2 | 50% activity with NADH compared to NADPH | Pseudomonas sp. | cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+ | - |
? | |
naphthalene + NADH + O2 | - |
Pseudomonas sp. | cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
naphthalene + NADH + O2 | initial oxidative reaction in bacterial naphthalene catabolism | Pseudomonas sp. | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulates cytochrome c reduction by ferredoxinNAP reductase | Pseudomonas sp. | |
FMN | stimulates cytochrome c reduction by ferredoxinNAP reductase | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | less than 50% of the activity with NADH | Pseudomonas sp. |