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Literature summary for 1.14.12.12 extracted from

  • Ensley, B.D.; Gibson, D.T.; Laborde, A.L.
    Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816 (1982), J. Bacteriol., 149, 948-954.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
10% ethanol and 10% glycerol are required for stability Pseudomonas sp.
DTT stabilizes Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pseudomonas sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Pseudomonas sp.
additional information no significant activation by the addition of Fe2+, Fe3+, Zn2+, Mg2+ or Cu2+ Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
naphthalene + NADH + O2 Pseudomonas sp. initial oxidative reaction in bacterial naphthalene catabolism (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography separates the reductase from the two other enzyme components Pseudomonas sp.
purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
naphthalene + NAD(P)H + O2 50% activity with NADH compared to NADPH Pseudomonas sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
?
naphthalene + NADH + O2
-
Pseudomonas sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
?
naphthalene + NADH + O2 initial oxidative reaction in bacterial naphthalene catabolism Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
FAD stimulates cytochrome c reduction by ferredoxinNAP reductase Pseudomonas sp.
FMN stimulates cytochrome c reduction by ferredoxinNAP reductase Pseudomonas sp.
NADH
-
Pseudomonas sp.
NADPH less than 50% of the activity with NADH Pseudomonas sp.