Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Delftia tsuruhatensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE | Delftia tsuruhatensis |
46000 | - |
alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE | Delftia tsuruhatensis |
235000 | - |
gel filtration | Delftia tsuruhatensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
terephthalate + NADH + O2 + H+ | Delftia tsuruhatensis | - |
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | - |
? | |
terephthalate + NADH + O2 + H+ | Delftia tsuruhatensis T7 | - |
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia tsuruhatensis | Q8GRC1 | - |
- |
Delftia tsuruhatensis T7 | Q8GRC1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Delftia tsuruhatensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
purified enzyme | Delftia tsuruhatensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
terephthalate + NADH + O2 + H+ | - |
Delftia tsuruhatensis | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | - |
? | |
terephthalate + NADH + O2 + H+ | - |
Delftia tsuruhatensis T7 | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE | Delftia tsuruhatensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | [2Fe-2S] Rieske center | Delftia tsuruhatensis | |
NADH | - |
Delftia tsuruhatensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Delftia tsuruhatensis | isoelectric focusing, beta subunit | - |
4.9 |
Delftia tsuruhatensis | isoelectric focusing, alpha subunit | - |
5.3 |