Protein Variants | Comment | Organism |
---|---|---|
A405G | synthesizes 17% 3-methylcatechol, 83% 4-methylcatechol from substrate 3-nitrotoluene | Acidovorax sp. |
I204A | synthesizes 2% 3-methylcatechol, 97% 4-methylcatechol and 1% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene | Acidovorax sp. |
I204T | synthesizes 5% 3-methylcatechol, 93% 4-methylcatechol and 2% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene | Acidovorax sp. |
I204V | synthesizes 13% 3-methylcatechol, 83% 4-methylcatechol and 5% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene | Acidovorax sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
2-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.003 | - |
2-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.0038 | - |
2-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
0.0039 | - |
3-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.0042 | - |
3-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
0.0045 | - |
nitrobenzene | pH 6.8, 30°C | Acidovorax sp. | |
0.0047 | - |
nitrobenzene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
0.0048 | - |
nitrobenzene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.0054 | - |
3-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.0057 | - |
2-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.0062 | - |
nitrobenzene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.0078 | - |
3-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.0092 | - |
3-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
0.0099 | - |
nitrobenzene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.0137 | - |
2-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
0.019 | - |
2-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
0.04 | - |
3-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
0.047 | - |
nitrobenzene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax sp. | - |
- |
- |
Acidovorax sp. JS42 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrotoluene + NADH + O2 | - |
Acidovorax sp. | 3-methylcatechol + nitrite + NAD+ | - |
? | |
2-nitrotoluene + NADH + O2 | - |
Acidovorax sp. JS42 | 3-methylcatechol + nitrite + NAD+ | - |
? | |
3-nitrotoluene + NADH + O2 | - |
Acidovorax sp. | 3-methylcatechol + nitrite + NAD+ | products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol | ? | |
3-nitrotoluene + NADH + O2 | - |
Acidovorax sp. JS42 | 3-methylcatechol + nitrite + NAD+ | products are 72% 3-methylcatechol, 25% 4-methylcatechol and 4% 3-nitrobenzyl alcohol | ? | |
3-nitrotoluene + NADH + O2 | - |
Acidovorax sp. | 4-methylcatechol + nitrite + NAD+ | sole product | ? | |
3-nitrotoluene + NADH + O2 | - |
Acidovorax sp. JS42 | 4-methylcatechol + nitrite + NAD+ | sole product | ? | |
4-nitrotoluene + NADH + O2 | - |
Acidovorax sp. | 4-methylcatechol + nitrite + NAD+ | - |
? | |
4-nitrotoluene + NADH + O2 | - |
Acidovorax sp. JS42 | 4-methylcatechol + nitrite + NAD+ | - |
? | |
additional information | all three mononitrotoluene isomers serve as substrates for 2-nitrotoluene dioxygenase, but strain JS42 is unable to grow on 3- or 4-nitrotoluene | Acidovorax sp. | ? | - |
? | |
additional information | all three mononitrotoluene isomers serve as substrates for 2-nitrotoluene dioxygenase, but strain JS42 is unable to grow on 3- or 4-nitrotoluene | Acidovorax sp. JS42 | ? | - |
? | |
nitrobenzene + NADH + O2 | - |
Acidovorax sp. | catechol + nitrite + NAD+ | - |
? | |
nitrobenzene + NADH + O2 | - |
Acidovorax sp. | catechol + nitrite + NAD+ | reaction of EC 1.14.12.23 | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
0.13 | - |
3-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.15 | - |
3-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
0.16 | - |
2-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
0.19 | - |
2-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.19 | - |
nitrobenzene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
0.21 | - |
3-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.21 | - |
2-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.22 | - |
nitrobenzene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
0.23 | - |
nitrobenzene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
0.25 | - |
nitrobenzene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.25 | - |
2-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.26 | - |
3-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
0.27 | - |
3-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
0.27 | - |
3-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
0.27 | - |
nitrobenzene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
0.3 | - |
nitrobenzene | pH 6.8, 30°C | Acidovorax sp. | |
0.34 | - |
2-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | Acidovorax sp. strain JS42 uses 2-nitrotoluene as a sole source of carbon and energy. The first enzyme of the degradation pathway, 2-nitrotoluene 2,3-dioxygenase, adds both atoms of molecular oxygen to 2-nitrotoluene, forming nitrite and 3-methylcatechol | Acidovorax sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
3-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
4 | - |
nitrobenzene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
6.5 | - |
2-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
12 | - |
2-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
17 | - |
3-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
22 | - |
nitrobenzene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
29 | - |
3-nitrotoluene | mutant I204A, pH 6.8, 30°C | Acidovorax sp. | |
37 | - |
nitrobenzene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. | |
37 | - |
2-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
39 | - |
3-nitrotoluene | mutant I204T, pH 6.8, 30°C | Acidovorax sp. | |
53 | - |
nitrobenzene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
58 | - |
nitrobenzene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
65 | - |
3-nitrotoluene | pH 6.8, 30°C | Acidovorax sp. | |
67 | - |
nitrobenzene | pH 6.8, 30°C | Acidovorax sp. | |
68 | - |
3-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
83 | - |
2-nitrotoluene | mutant I204V, pH 6.8, 30°C | Acidovorax sp. | |
90 | - |
2-nitrotoluene | wild-type, pH 6.8, 30°C | Acidovorax sp. | |
160 | - |
2-nitrotoluene | mutant A405G, pH 6.8, 30°C | Acidovorax sp. |