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Literature summary for 1.14.12.7 extracted from
- Phthalate dioxygenase (1990), Methods Enzymol., 188, 61-70.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.125 | - |
O2 | - |
Burkholderia cepacia |  |
| 0.3 | - |
Phthalate | 25°C | Burkholderia cepacia | |
| 0.5 | - |
Phthalate | 4°C | Burkholderia cepacia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | one Fe2+ is bound at a mononuclear site | Burkholderia cepacia | |
| Iron | two iron atoms are bound in a Rieske-type [2Fe-2S] center | Burkholderia cepacia | |
| additional information | optimal activity at ionic strength of 80 mM | Burkholderia cepacia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
DB01 | - |
| Burkholderia cepacia | - |
two separate proteins are required for phthalate dioxygenase activity: phthalate dioxygenase and phthalate dioxygenase reductase | - |
| Burkholderia cepacia DB01 | - |
DB01 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phthalate + NADH + H+ + O2 = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ | reduced phthalate dioxygenase reductase first interacts with phthylate dioxygenase and reduces the Rieske [2Fe-2S]center. Phthalate and O2 then bind reversibly and reduced phthalate dioxygenase transfers one more electron to the enzyme | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.31 | - |
- |
Burkholderia cepacia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8.0, 100 mM HEPES buffer, 20% v/v glycerol, stable for 1 week | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phthalate + NADH + O2 | - |
Burkholderia cepacia | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? | |
| phthalate + NADH + O2 | - |
Burkholderia cepacia DB01 | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, SDS-PAGE | Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
pH 6.8 or pH 8.0, 12 h stable | Burkholderia cepacia |
| 42 | - |
1 h, stable | Burkholderia cepacia |
| 60 | - |
5 min, stable | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.2 | - |
Burkholderia cepacia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
room temperature, 12 h, stable | Burkholderia cepacia |
| 8 | - |
room temperature, 12 h, stable | Burkholderia cepacia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | specific for NADH | Burkholderia cepacia | |
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Release 2023.1 (January 2023)
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