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Literature summary for 1.14.12.7 extracted from
- Distal end of 105-125 loop - a putative reductase binding domain of phthalate dioxygenase (2009), Arch. Biochem. Biophys., 487, 10-18.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli 41 (DE3) | Burkholderia cepacia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H120A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
| K117A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
| K117A/K119A/H120A/K121A/Y123A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
| K119A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
| K121A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
| W94A | adjacent to the Rieske center | Burkholderia cepacia |
| W94F | adjacent to the Rieske center | Burkholderia cepacia |
| W94Y | adjacent to the Rieske center | Burkholderia cepacia |
| Y123A | putative participation in phthalate dioxygenase reductase binding | Burkholderia cepacia |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Rieske 2Fe-2S center | Burkholderia cepacia |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
| Burkholderia cepacia DB01 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phthalate + NADH + H+ + O2 | - |
Burkholderia cepacia | cis-4,5-dihydro-4,5-dihydroxyphthalate + NAD+ | - |
? | |
| phthalate + NADH + H+ + O2 | - |
Burkholderia cepacia DB01 | cis-4,5-dihydro-4,5-dihydroxyphthalate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | - |
Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phthalate dioxygenase | - |
Burkholderia cepacia |
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Release 2023.1 (January 2023)
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