Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 blue | Paenarthrobacter nicotinovorans |
General Stability | Organism |
---|---|
dialysis against 1.5 M guanidinium hydrochloride removes FAD, no renaturation possible | Paenarthrobacter nicotinovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydroxypyridine | 100% inhibition at 0.1 mM, irreversible inhibitor | Paenarthrobacter nicotinovorans | |
2,6-dihydroxynicotinamide | 55% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans | |
2,6-dimethoxypyridine | 100% inhibition at 0.1 mM, irreversible inhibitor | Paenarthrobacter nicotinovorans | |
2-Hydroxypyridine | 48% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans | |
resorcine | 51% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
2,6-Dihydroxypyridine | pH 8, 20°C | Paenarthrobacter nicotinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dihydroxypyridine + NADH + O2 | Paenarthrobacter nicotinovorans | - |
2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
Purification (Comment) | Organism |
---|---|
- |
Paenarthrobacter nicotinovorans |
Renatured (Comment) | Organism |
---|---|
after removal of FAD through precipitation with 50% (NH4)2SO4 at pH 2.0, incubation with 0.1 mM FAD leeds to 90% activity recovery | Paenarthrobacter nicotinovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? | |
2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | product dimerizes spontaneously in the presence of oxygen to form a blue pigment | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Paenarthrobacter nicotinovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Paenarthrobacter nicotinovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable | Paenarthrobacter nicotinovorans |
52 | - |
inactivation | Paenarthrobacter nicotinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol FAD per dimer | Paenarthrobacter nicotinovorans | |
NADH | no reaction with NADPH | Paenarthrobacter nicotinovorans |