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Literature summary for 1.14.13.122 extracted from

  • Eggink, L.L.; LoBrutto, R.; Brune, D.C.; Brusslan, J.; Yamasato, A.; Tanaka, A.; Hoober, J.K.
    Synthesis of chlorophyll b: localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit (2004), BMC Plant Biol., 4, 5.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.122

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli in membranes, phylogenetic tree Arabidopsis thaliana
expression of GFP-tagged enzyme in Chlamydomonas reinhardtii cells, phylogenetic tree Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast envelope inner membrane Arabidopsis thaliana 9507
-
chloroplast envelope inner membrane, in light and dark grown cells Chlamydomonas reinhardtii 9507
-
thylakoid membrane
-
 Arabidopsis thaliana 42651
-
thylakoid membrane only in light grown cells Chlamydomonas reinhardtii 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear non-hem iron-binding site Chlamydomonas reinhardtii
Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear non-hem iron-binding site Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-hydroxychlorophyllide a + O2 + NADPH Chlamydomonas reinhardtii
-
 chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH Arabidopsis thaliana
-
 chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH Chlamydomonas reinhardtii cw15
-
 chlorophyllide b + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+ Chlamydomonas reinhardtii
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+ Arabidopsis thaliana
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+ Chlamydomonas reinhardtii cw15
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
additional information Chlamydomonas reinhardtii chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?
additional information Arabidopsis thaliana chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?
additional information Chlamydomonas reinhardtii cw15 chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii cw15
-
-
-

Reaction

Reaction Comment Organism Reaction ID
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction Chlamydomonas reinhardtii
a
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr518 which is involved in the catalytic reaction Arabidopsis thaliana
a
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction Chlamydomonas reinhardtii
a
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr518 which is involved in the catalytic reaction Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-hydroxychlorophyllide a + O2 + NADPH
-
 Chlamydomonas reinhardtii chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH
-
 Arabidopsis thaliana chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH
-
 Chlamydomonas reinhardtii cw15 chlorophyllide b + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Chlamydomonas reinhardtii 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Chlamydomonas reinhardtii cw15 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
additional information chlorophyll b is required for assembly of the light-harvesting complexes Chlamydomonas reinhardtii ?
-
 ?
additional information chlorophyll b is required for assembly of the light-harvesting complexes Arabidopsis thaliana ?
-
 ?
additional information chlorophyll b is required for assembly of the light-harvesting complexes Chlamydomonas reinhardtii cw15 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CAO
-
 Chlamydomonas reinhardtii
CAO
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Chlamydomonas reinhardtii
NADPH
-
 Arabidopsis thaliana