Cloned (Comment) | Organism |
---|---|
expressed in Trichoplusia ni cells and in Escherichia coli strain XL-1 Blue | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-linked glycosylation at Asn61 and O-linked glycosylation at Thr29, Thr249, and Thr381. Posttranslational modification of human FMO3 by insect cells is limited to cleavage at the N-terminal methionine | Homo sapiens |
Purification (Comment) | Organism |
---|---|
cholate solubilization and sequential column chromatography on octyl-Sepharose, DEAE-Sepharose, and hydroxyapatite | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | FMO3 is quantitatively a major human liver monooxygenase | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.049 | - |
crude enzyme from membranes, using methyl 4-tolyl sulfide as substrate, in 25 mM potassium diphosphate (pH 8.5), at 37°C | Homo sapiens |
0.201 | - |
after purification, using methyl 4-tolyl sulfide as substrate, in 25 mM potassium diphosphate (pH 8.5), at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl 4-tolyl sulfide + NADPH + H+ + O2 | - |
Homo sapiens | methyl 4-tolyl sulfoxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FMO3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | required for activity | Homo sapiens |