Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoyl-CoA | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
O2 | at pH 8.0 and 30°C | Pseudomonas sp. | |
0.006 | - |
epoxyphenylacetyl-CoA | at pH 8.0 and 30°C | Pseudomonas sp. | |
0.023 | - |
NADPH | at pH 8.0 and 30°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains a catalytic di-iron center | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. Y2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxyphenylacetyl-CoA + O2 + NADPH + H+ | the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up | Pseudomonas sp. | ? | - |
? | |
epoxyphenylacetyl-CoA + O2 + NADPH + H+ | the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up | Pseudomonas sp. Y2 | ? | - |
? | |
additional information | he non-physiological substrate benzoyl-CoA is not transformed to detectable products | Pseudomonas sp. | ? | - |
? | |
additional information | he non-physiological substrate benzoyl-CoA is not transformed to detectable products | Pseudomonas sp. Y2 | ? | - |
? | |
phenylacetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas sp. | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+ | - |
? | |
phenylacetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas sp. Y2 | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PaaABCE | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas sp. | |
NADPH | - |
Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | bifunctional PaaABCE acts as a phenylacetyl-CoA ring-1,2-epoxidase/ring-1,2-epoxyphenylacetyl-CoA deoxygenase | Pseudomonas sp. |