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Literature summary for 1.14.13.149 extracted from

  • Teufel, R.; Friedrich, T.; Fuchs, G.
    An oxygenase that forms and deoxygenates toxic epoxide (2012), Nature, 483, 359-362.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
benzoyl-CoA
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
O2 at pH 8.0 and 30°C Pseudomonas sp.
0.006
-
epoxyphenylacetyl-CoA at pH 8.0 and 30°C Pseudomonas sp.
0.023
-
NADPH at pH 8.0 and 30°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains a catalytic di-iron center Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. Y2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxyphenylacetyl-CoA + O2 + NADPH + H+ the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up Pseudomonas sp. ?
-
?
epoxyphenylacetyl-CoA + O2 + NADPH + H+ the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up Pseudomonas sp. Y2 ?
-
?
additional information he non-physiological substrate benzoyl-CoA is not transformed to detectable products Pseudomonas sp. ?
-
?
additional information he non-physiological substrate benzoyl-CoA is not transformed to detectable products Pseudomonas sp. Y2 ?
-
?
phenylacetyl-CoA + O2 + NADPH + H+
-
Pseudomonas sp. 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
?
phenylacetyl-CoA + O2 + NADPH + H+
-
Pseudomonas sp. Y2 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
?

Synonyms

Synonyms Comment Organism
PaaABCE
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.

General Information

General Information Comment Organism
metabolism bifunctional PaaABCE acts as a phenylacetyl-CoA ring-1,2-epoxidase/ring-1,2-epoxyphenylacetyl-CoA deoxygenase Pseudomonas sp.