Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one | pH 9.0, 20°C | Pseudomonas putida | |
0.127 | - |
Cyclobutanone | pH 9.0, 20°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
63500 | - |
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | H3JQW0 | - |
- |
Pseudomonas putida NCIMB 10007 | H3JQW0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2200 | - |
pH 9.0, 20°C | Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 24 h, 50% loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+ | - |
Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 34% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 34% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
2-cyclohexen-1-one + O2 + NADPH + H+ | 74% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
2-cyclohexen-1-one + O2 + NADPH + H+ | 74% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
2-cyclopenten-1-one + O2 + NADPH + H+ | 62% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 22% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 22% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
3-methyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
cyclobutanone + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
norcamphor + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
half-life 196 min | Pseudomonas putida |
25 | - |
half-life 43 min | Pseudomonas putida |
30 | - |
half-life 9 min | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
Cyclobutanone | pH 9.0, 20°C | Pseudomonas putida | |
49.3 | - |
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one | pH 9.0, 20°C | Pseudomonas putida |