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Literature summary for 1.14.13.166 extracted from

  • Perry, L.; Zylstra, G.
    Cloning of a gene cluster involved in the catabolism of p-nitrophenol by Arthrobacter sp. strain JS443 and characterization of the p-nitrophenol monooxygenase (2007), J. Bacteriol., 189, 7563-7572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.166

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. A7YVV2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-nitrophenol-grown cell
-
 Arthrobacter sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,5-trichlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2,5-dichlorohydroquinone + 5-chloro-2-hydroxyquinol + ? + NAD+ + H2O
-
 ?
2,4,6-trichlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2,6-dichlorohydroquinone + 6-chloro-2-hydroxyquinol + ? + NAD+ + H2O
-
 ?
2,4-dinitrophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. 2-nitrohydroquinone + nitrite + NAD+ + H2O
-
 ?
3-chlorophenol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
3-cresol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
3-nitrophenol + NADH + H+ + O2
-
 Arthrobacter sp. 3-nitrohydroquinone + NAD+ + H2O
-
 ?
4-chlorocatechol + NADH + H+ + O2 good substrate Arthrobacter sp. 5-chlorohydroxyquinol + ? + NAD+ + H2O
-
 ?
4-chlorophenol + NADH + H+ + O2
-
 Arthrobacter sp. 1,2,4-trihydroxybenzene + 2-hydroxy-1,4-benzoquinone + ? + NAD+ + H2O
-
 ?
4-chlororesorcinol + NADH + H+ + O2 good substrate Arthrobacter sp. 1,2,4-trihydroxybenzene + ? + NAD+ + H2O
-
 ?
4-cresol + NADH + H+ + O2 poor substrate Arthrobacter sp. 4-hydroxy-4-methyl-2,5-cyclohexadien-1-one + NAD+ + H2O
-
 ?
4-methylcatechol + NADH + H+ + O2 poor substrate Arthrobacter sp. ? + NAD+ + H2O
-
 ?
4-nitrocatechol + NADH + H+ + O2 good substrate Arthrobacter sp. 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
 ?
4-nitrophenol + NADH + H+ + O2 best substrate Arthrobacter sp. 2-hydroxy-1,4-benzoquinone + 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
additional information hydroquinone, resorcinol, and catechol are not substrates for NpdA2 Arthrobacter sp. ?
-
 ?
phenol + NADH + H+ + O2 poor substrate Arthrobacter sp. hydroquinone + ? + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NpdA2
-
 Arthrobacter sp.
p-nitrophenol monooxygenase
-
 Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arthrobacter sp.
NADH
-
 Arthrobacter sp.