Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.187 extracted from

  • Hu, Y.; Al-Mestarihi, A.; Grimes, C.L.; Kahne, D.; Bachmann, B.O.
    A unifying nitrososynthase involved in nitrosugar biosynthesis (2008), J. Am. Chem. Soc., 130, 15756-15757.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 Micromonospora carbonacea overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O
-
?
dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 Micromonospora carbonacea var. africana overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O
-
?

Organism

Organism UniProt Comment Textmining
Micromonospora carbonacea
-
-
-
Micromonospora carbonacea var. africana
-
-
-

Reaction

Reaction Comment Organism Reaction ID
dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 = dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O (overall reaction) Micromonospora carbonacea
dTDP-beta-L-evernosamine + NADPH + H+ + O2 = dTDP-N-hydroxy-beta-L-evernosamine + NADP+ + H2O (1a) Micromonospora carbonacea
dTDP-N-hydroxy-beta-L-evernosamine + NADPH + H+ + O2 = dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + NADP+ + 2 H2O (1b) Micromonospora carbonacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin Micromonospora carbonacea dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O
-
?
dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin Micromonospora carbonacea var. africana dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O
-
?
dTDP-beta-L-evernosamine + NADPH + H+ + O2 dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose Micromonospora carbonacea dTDP-N-hydroxy-beta-L-evernosamine + NADP+ + H2O
-
?
dTDP-beta-L-evernosamine + NADPH + H+ + O2 dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose Micromonospora carbonacea var. africana dTDP-N-hydroxy-beta-L-evernosamine + NADP+ + H2O
-
?
dTDP-N-hydroxy-beta-L-evernosamine + NADPH + H+ + O2 dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose Micromonospora carbonacea dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + NADP+ + 2 H2O
-
?
dTDP-N-hydroxy-beta-L-evernosamine + NADPH + H+ + O2 dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose Micromonospora carbonacea var. africana dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + NADP+ + 2 H2O
-
?

Cofactor

Cofactor Comment Organism Structure
FAD required Micromonospora carbonacea
NADPH
-
Micromonospora carbonacea