Cloned (Comment) | Organism |
---|---|
expression in Streptomyces albus or in Escherichia coliBö21(DE3) | Streptomyces sahachiroi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
NADPH | pH 9.0, 25°C | Streptomyces sahachiroi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyl-1-naphthoate + NADPH + H+ + O2 | Streptomyces sahachiroi | the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B | 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sahachiroi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sahachiroi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyl-1-naphthoate + NADPH + H+ + O2 | - |
Streptomyces sahachiroi | 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O | - |
? | |
5-methyl-1-naphthoate + NADPH + H+ + O2 | the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B | Streptomyces sahachiroi | 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AziB1 | - |
Streptomyces sahachiroi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | pH 9.0, 25°C | Streptomyces sahachiroi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces sahachiroi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 45% of maximal activity, pH 10.0: about 80% of maximal activity | Streptomyces sahachiroi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | a heme-thiolate protein (P450) | Streptomyces sahachiroi |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B | Streptomyces sahachiroi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
NADPH | pH 9.0, 25°C | Streptomyces sahachiroi |