Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.189 extracted from

  • Ding, W.; Deng, W.; Tang, M.; Zhang, Q.; Tang, G.; Bi, Y.; Liu, W.
    Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B (2010), Mol. Biosyst., 6, 1071-1081.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces albus or in Escherichia coliBö21(DE3) Streptomyces sahachiroi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
NADPH pH 9.0, 25°C Streptomyces sahachiroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-methyl-1-naphthoate + NADPH + H+ + O2 Streptomyces sahachiroi the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sahachiroi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sahachiroi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyl-1-naphthoate + NADPH + H+ + O2
-
Streptomyces sahachiroi 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
-
?
5-methyl-1-naphthoate + NADPH + H+ + O2 the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B Streptomyces sahachiroi 3-hydroxy-5-methyl-1-naphthoate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
AziB1
-
Streptomyces sahachiroi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
NADPH pH 9.0, 25°C Streptomyces sahachiroi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces sahachiroi

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 45% of maximal activity, pH 10.0: about 80% of maximal activity Streptomyces sahachiroi

Cofactor

Cofactor Comment Organism Structure
additional information a heme-thiolate protein (P450) Streptomyces sahachiroi

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of 3-methoxy-5-methyl-1-naphthoate a component of of the the antitumor antibiotic azinomycin B Streptomyces sahachiroi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0054
-
NADPH pH 9.0, 25°C Streptomyces sahachiroi