Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-lysine | nonsubstrate activator stimulating NADPh oxidation 3.9fold at 2 mM causing H2O2 formation, overview | Pseudomonas aeruginosa | |
L-ornithine | activates 5fold | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene pvdA, expression of His-tagged enzyme in Escherichia coli strain Rosetta2(DE3) | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminopentanoic acid | competitive inhibition | Pseudomonas aeruginosa | |
L-2,4-diaminobutyrate | competitive inhibition | Pseudomonas aeruginosa | |
L-homoserine | competitive inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetic analysis, overview | Pseudomonas aeruginosa | |
0.0219 | - |
FAD | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
0.058 | - |
L-ornithine | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
0.16 | - |
NADPH | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
136600 | - |
recombinant His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene pvdA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Pseudomonas aeruginosa |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
NADPH oxidase activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | no activity with D-ornithine | Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
additional information | the NADPH oxidase activity of the enzyme is tightly coupled to hydroxylamine formation | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer or pentamer | x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
L-ornithine N5-hydroxylase | - |
Pseudomonas aeruginosa |
PvdA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH profile, overview | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | specific for | Pseudomonas aeruginosa | |
additional information | no activity with FMN and NADH | Pseudomonas aeruginosa | |
NADPH | specific for | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
2.9 | - |
5-aminopentanoic acid | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
3.1 | - |
L-homoserine | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa | |
8.4 | - |
L-2,4-diaminobutyrate | pH 8.0, 25°C, recombinant detagged enzyme | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in pyoverdine siderophore biosynthesis, pyoverdine is required for acquiration of the essential iron from the host | Pseudomonas aeruginosa |