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Literature summary for 1.14.13.196 extracted from

  • Chocklett, S.W.; Sobrado, P.
    Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor (2010), Biochemistry, 49, 6777-6783.
    View publication on PubMed
Search for more literature for 1.14.13.196

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion to MBP containing an 8His tag at the N-terminus in Escherichia coli BL21T1R cells Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
NADH substrate inhibition Aspergillus fumigatus
NADP+ competitive inhibitor with respect to NADPH Aspergillus fumigatus
NADPH substrate inhibition Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
0.21
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
0.5
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
0.9
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
0.94
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.7
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.7
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
2
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Aspergillus fumigatus
57210
-
 4 * 57210, MALDI-TOF MS Aspergillus fumigatus
228000
-
 gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + NAD(P)H + H+ + O2 Aspergillus fumigatus the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis N5-hydroxy-L-ornithine + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q5SE95
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Reaction

Reaction Comment Organism Reaction ID
L-ornithine + NAD(P)H + H+ + O2 = N5-hydroxy-L-ornithine + NAD(P)+ + H2O the enzyme forms a ternary complex with NADP and L-ornithine during catalysis. The enzyme likely proceeds by a sequential kinetic mechanism Aspergillus fumigatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NAD(P)H + H+ + O2 the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD(P)+ + H2O
-
 ?
L-ornithine + NADH + H+ + O2 AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Aspergillus fumigatus
tetramer 4 * 57210, MALDI-TOF MS Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
AfSidA ambiguous Aspergillus fumigatus
siderophore A
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
0.5
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
0.63
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
0.68
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
0.87
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus
1.18
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.25
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.36
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Aspergillus fumigatus
NADH 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus
NADPH 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
 NADH pH 7.5, 25°C Aspergillus fumigatus
2.8
-
 NADPH pH 7.5, 25°C Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.28
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
0.29
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
0.43
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
1.3
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.3
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.33
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus
3.4
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
21.05
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus