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Literature summary for 1.14.13.2 extracted from

  • Hosokawa, K.; Stanier, R.Y.
    Crystallization and properties of p-hydroxybenzoate hydroxylase from Pseudomonas putida (1966), J. Biol. Chem., 241, 2453-2460.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxybenzoate above 1 mM Pseudomonas fluorescens
4-Aminobenzoate above 1 mM Pseudomonas fluorescens
4-Fluorobenzoate above 1 mM Pseudomonas fluorescens
4-hydroxybenzoate above 1 mM Pseudomonas fluorescens
benzoate above 1 mM Pseudomonas fluorescens
Fe2+
-
Pseudomonas fluorescens
protocatechuate above 1 mM Pseudomonas fluorescens
salicylate above 1 mM Pseudomonas fluorescens
Zn2+ 0.2 mM, reversed by 10 mM glutathione Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0213
-
4-hydroxybenzoate
-
Pseudomonas fluorescens
0.0227
-
NADPH reaction with 4-hydroxybenzoate Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
gel filtration Pseudomonas fluorescens
83600
-
low speed sedimentation without reaching equilibrium Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + O2 Pseudomonas fluorescens inducible enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzoate + NADPH + O2 1.5% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
?
3-bromo-4-hydroxybenzoate + NADPH + O2 3.2% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2 inducible enzyme Pseudomonas fluorescens ?
-
?
4-toluate + NADPH + O2 0.29% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
benzene sulfonate + NADPH + O2 0.34% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
additional information under anaerobic conditions, the enzyme can catalyze a reduction of FAD by NADPH provided that 4-hydroxybenzoate is present Pseudomonas fluorescens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.7
-
4-hydroxybenzoate
-
Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
FAD contains approximately 0.8 mol FAD per mol of enzyme Pseudomonas fluorescens
NADPH no activity with NADH Pseudomonas fluorescens