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Literature summary for 1.14.13.2 extracted from

  • Wang, J.; Ortiz-Maldonado, M.; Entsch, B.; Massey, V.; Ballou, D.; Gatti, D.L.
    Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase (2002), Proc. Natl. Acad. Sci. USA, 99, 608-613.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion, hanging drops containing 4 mg/ml protein, 100 mM potassium phosphate, pH 7.0, 0.05 mM glutathione, 30 mM sodium sulfite, 0.02 mM FAD, 450 mM ammonium sulfate are equilibrated at 30°C for 7-10 days against a well solution of similar composition, but containing 900 mM ammonium sulfate, crystals of R220Q PHBH diffract to approx. 2.0 A Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
R220Q 1% of wild-type activity, lower affinity to 4-hydroxybenzoate than wild-type Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + O2 Pseudomonas aeruginosa
-
protocatechuate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P20586
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
PHBH
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas aeruginosa
NADPH
-
Pseudomonas aeruginosa