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Literature summary for 1.14.13.20 extracted from

  • Radjendirane, V.; Bhat, M.A.; Vaidyanathan, C.S.
    Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia [published erratum appears in Arch Biochem Biophys 1992 Jul;296(1):354] (1991), Arch. Biochem. Biophys., 288, 169-176.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,4,5-Trichlorophenol
-
Burkholderia cepacia
2,4,6-Trichlorophenol
-
Burkholderia cepacia
guanidine hydrochloride
-
Burkholderia cepacia
Hg2+
-
Burkholderia cepacia
p-chloromercuribenzoate
-
Burkholderia cepacia
SDS
-
Burkholderia cepacia
Urea
-
Burkholderia cepacia
Zn2+
-
Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
2,4-Dichlorophenol
-
Burkholderia cepacia
0.1
-
NADPH
-
Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
alpha4, 4 * 69000, SDS-PAGE Burkholderia cepacia
275000
-
gel filtration and ultracentrifugation Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorophenol + NADPH + O2 Burkholderia cepacia
-
3,5-dichlorocatechol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
single step affinity chromatography Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
-
Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + NADPH + O2
-
Burkholderia cepacia 3,5-dichlorocatechol + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 69000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Burkholderia cepacia
FAD 1 mol of enzyme contains: 4 mol FAD Burkholderia cepacia
FAD requires exogenous addition of FAD Burkholderia cepacia
NADH NADPH preferred to NADH Burkholderia cepacia
NADPH
-
Burkholderia cepacia