Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,5-Trichlorophenol | - |
Burkholderia cepacia | |
2,4,6-Trichlorophenol | - |
Burkholderia cepacia | |
guanidine hydrochloride | - |
Burkholderia cepacia | |
Hg2+ | - |
Burkholderia cepacia | |
p-chloromercuribenzoate | - |
Burkholderia cepacia | |
SDS | - |
Burkholderia cepacia | |
Urea | - |
Burkholderia cepacia | |
Zn2+ | - |
Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2,4-Dichlorophenol | - |
Burkholderia cepacia | |
0.1 | - |
NADPH | - |
Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
alpha4, 4 * 69000, SDS-PAGE | Burkholderia cepacia |
275000 | - |
gel filtration and ultracentrifugation | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | Burkholderia cepacia | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
single step affinity chromatography | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | - |
Burkholderia cepacia | 3,5-dichlorocatechol + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 69000, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Burkholderia cepacia | |
FAD | 1 mol of enzyme contains: 4 mol FAD | Burkholderia cepacia | |
FAD | requires exogenous addition of FAD | Burkholderia cepacia | |
NADH | NADPH preferred to NADH | Burkholderia cepacia | |
NADPH | - |
Burkholderia cepacia |